IL8 CLIA Kit for Bovine(Bos taurus) | EIAab.comIL8 CLIA Kit for Bovine(Bos taurus)

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Index > Protein center > IL8(Gene name) > Bovine

IL8 (Gene name),

Interleukin-8 (Protein name ), IL8_BOVIN from NCBI database.

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General Annotation

Antigen Annotation

Predicted Eptitope

Vaild Sequence

Related Databases

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Gene name:

IL8(CXCL8);

Protein name:

Interleukin-8(IL-8);

Alternative:

C-X-C motif chemokine 8;

Organism:

Bovine (Bos taurus).

General Annotation

Sub Unit:

Homodimer.

Function:

IL-8 is a chemotactic factor that attracts neutrophils, basophils, and T-cells, but not monocytes. It is also involved in neutrophil activation. It is released from several cell types in response to an inflammatory stimulus.

Subcellular Location:

Secreted

Protein Attributes:

Sequence length:

101

Sequence:

50:

100:

101:

3D Structure:

N/A

Predicted Eptitope:

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Vaild Sequence:

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Related Databases

UniGene:

Bt.49740

SMR:

P79255

KEGG:

bta:280828

Pfam:

PF00048

String:

P79255

Uniprot:

P79255

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ELISA Kit for Bovine IL-8

Cat.:

E0080c

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96T

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ELISA Kit for Bovine IL-8

Cat.:

E0080p

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96T

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ELISA Kit for Bovine IL-8

Cat.:

E0080h

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96T

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ELISA Kit for Bovine IL-8

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ELISA Kit for Bovine IL-8

Cat.:

E0080d

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96T

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ELISA Kit for Bovine IL-8

Cat.:

E0080b

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96T

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CLIA Kit for Bovine IL-8

Cat.:

U0080b

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96T

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CLIA Kit for Bovine IL-8

Cat.:

U0080d

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Looking for manual.

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96T

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CLIA Kit for Bovine IL-8

Cat.:

U0080c

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Manual:

Looking for manual.

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Packing:

96T

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Please sign in first.

CLIA Kit for Bovine IL-8

Cat.:

U0080h

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Looking for manual.

MSDS:

Please sign in first.

Packing:

96T

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CLIA Kit for Bovine IL-8

Cat.:

U0080p

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CLIA Kit for Bovine IL-8

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Polyclonal Antibody for Bovine IL-8

Cat.:

P0080Rb-h

Price:

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Packing:

40ug/0.2ml

Monoclonal Antibody for Bovine IL-8

Protein for Bovine IL-8

Cat.:

R0080h

Price:

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Packing:

1mg

Purity:

>90%

Protein for Bovine IL-8

R&D Technical Data

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

12% SDS-PAGE Analysis

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤4.2%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤7.5%

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤ 6.3%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤ 8.6%

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤4.1%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤7.5%

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Recovery

Recovery was determined by spiking various levels of IL8 into serum and plasma .

Sample Type	Average(%)	Recovery Range(%)
Serum	100	97-106
Plasma	101	95-104

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Recovery was determined by spiking various levels of ACE into serum and plasma .

Sample Type	Average(%)	Recovery Range(%)
Serum	99	93-105
Plasma	98	94-106

Recovery was determined by spiking various levels of IL8 into serum and plasma .

Sample Type	Average(%)	Recovery Range(%)
Serum	105	100-106
Plasma	104	102-107

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of IL8 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	100-105%	97-104%	95-101%	103-106%
EDTA plasma(n=5)	98-104%	94-103%	90-97%	105-109%
heparin plasma(n=5)	94-99%	103-108%	96-104%	98-105%

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ACE and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	91-103%	92-105%	95-106%	94-109%
EDTA plasma(n=5)	89-95%	93-103%	94-102%	95-106%
heparin plasma(n=5)	93-105%	94-107%	101-106%	96-108%

Sample	1：2	1:4	1:8	1:16
serum(n=5)	98-102%	103-107%	97-105%	96-103%
EDTA plasma(n=5)	100-104%	99-107%	102-108%	97-102%
heparin plasma(n=5)	104-106%	94-102%	108-112%	97-103%

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

References

1.

"Molecular cloning and expression of bovine interleukin-8."
Morsey M.A. , Popowych Y. , Kowalski J. , Gerlach G. , Godson D. , Campos M. , Babiuk L.A.
Microb. Pathog.20:203-212(1996) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA]
2.

"Cloning and sequencing of bovine interleukin 8 cDNA isolated from lipopolysaccharide stimulated monocytes in vitro."
Galligan C.L. , Yoshimura T. , Coomber B.L.
Submitted (2000-02) to the EMBL/GenBank/DDBJ databases

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA]
3.

"Estimating probability of parentage in U.S. beef and dairy cattle with single nucleotide polymorphisms."
Heaton M.P. , Clawson M.L. , Snelling W.M. , Keele J.W. , Harhay G.P. , Wiedmann R.T. , Bennett G.L. , Smith T.P.L. , Stone R.T. , Freking B.A. , Van Tassell C.P. , Sonstegard T.S. , Gasbarre L.C. , Carr J. , DiBello P. , Kumar M. , Lee M.S. , Murthy J. , more...
Submitted (2004-12) to the EMBL/GenBank/DDBJ databases

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]
4.

"U.S. veterinary immune reagent network: expressed bovine gene sequences."
U.S. Veterinary Immune Reagent Network , Hudgens T. , Tompkins D. , Baldwin C.L.
Submitted (2007-11) to the EMBL/GenBank/DDBJ databases

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]
strain: Belted Galloway.
tissue: Peripheral blood.
5.

NIH - Mammalian Gene Collection (MGC) project
Submitted (2005-08) to the EMBL/GenBank/DDBJ databases

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]
strain: Hereford.
tissue: Ascending colon.

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